How the HPLC Method works

How the HPLC method works in protein certification

In the world of protein supplements, accuracy and reliability in label information is essential to ensure quality and consumer safety. Here at PRO QUALITY, we use High Performance Liquid Chromatography (HPLC) to offer detailed and reliable certification of protein products.

But what exactly is HPLC and why is it such a valued technique in food science?

What is HPLC?

It is an advanced analytical technique used to separate, identify and quantify each component found in a mixture. In the context of protein supplements, it allows precise analysis of protein composition, identifying not only the types of proteins present, but also their quality and purity.

How is HPLC Applied in Protein Certification?

Sample preparation

We begin by preparing the protein sample, making sure it is in the proper format for analysis.

Sample analysis

The prepared sample is injected into the HPLC system. Through a specialized column, proteins are separated based on their physicochemical properties.

Detection and quantification

As the proteins are separated, they pass through a detector that identifies and quantifies each component. This step is crucial to determine the purity and quality of the protein.

Results comparison

The results obtained are meticulously compared to the specifications on the product label. This allows us to verify whether the declared composition matches the actual composition of the product.

To do this, we must take into account the margin of uncertainty that will depend on the chemical compound with which the proteins are diluted before performing the analysis to determine the amount of nitrogen there is and consequently the amount of total protein, therefore each Laboratory has its own uncertainty, all very similar, but different depending on the laboratory. In our case, it is 2%.

Why is HPLC reliable?

HPLC is a highly precise technique that provides detailed results on protein composition. This precision is essential to ensure that products meet quality and safety standards. Additionally, HPLC is capable of detecting adulterations and contaminants that other techniques may miss, ensuring an additional level of protection for consumers.

Conclusion

Confidence in protein supplements begins with knowing that what the label says is exactly what is on the bottle. By using HPLC technology, PRO QUALITY offers that certainty. We ensure that each product that bears our seal has been rigorously tested and verified, providing consumers with the quality and safety they deserve.

At PRO QUALITY, we are committed to maintaining the highest standards of transparency and quality in the supplement industry. Thanks to HPLC and our expert team, we can provide protein certification you can trust.